Software Tools :: Mapping and Digests

Background

Mapping and Digests

Programs

Restriction enzymes

  • GCG Programs
    • Map: Finds restriction enzyme cut sites
    • MapPlot: Displays restriction sites graphically
    • MapSort: Displays fragments from a single or double digest of a nucleic acid sequence
  • EMBOSS Programs
    • restrict: Finds restriction enzyme cleavage sites
    • remap: Displays a sequence with restriction cut sites, translation etc
    • restover: Finds restriction enzymes that produce a specific overhang
    • silent: Silent mutation restriction enzyme scan
    • recoder: Removes restriction sites but maintains the same translation

Proteolytic enzymes

  • GCG Programs
    • PeptideMap: Finds proteolytic enzyme cut sites
    • PeptideSort: Displays peptide fragments from a digest of a protein sequence
  • EMBOSS Programs
    • digest: Protein proteolytic enzyme or reagent cleavage digest

Isoelectric points and charge plots

  • GCG Programs
    • Isoelectric: Plots the charge as a function of pH for a peptide sequence
  • EMBOSS Programs
    • charge: Protein charge plot
    • iep: Calculates the isoelectric point of a protein

Related Tools

  • EMBOSS Programs
    • redata: Search REBASE for enzyme name, references, suppliers etc

This website will look much better in a browser that supports web standards, but it has been designed so that it is still usable and accessible to any browser or web-enabled device.